細(xì)胞名稱(chēng)

人急性T淋巴細(xì)胞白血病細(xì)胞 Jurkat

貨物編碼

BFN60807355

產(chǎn)品規(guī)格

T25培養(yǎng)瓶x1

1.5ml凍存管x2

細(xì)胞數(shù)量

1x10^6

1x10^6

保存溫度

37℃

-198℃

運(yùn)輸方式

常溫保溫運(yùn)輸

干冰運(yùn)輸

安全等級(jí)

1

用途限制

僅供科研           1類(lèi)

培養(yǎng)體系

DMEM高糖培養(yǎng)基+10%FBS+1%雙抗 

培養(yǎng)溫度

37℃

二氧化碳濃度

5%

簡(jiǎn)介

人急性T淋巴細(xì)胞白血病細(xì)胞Jurkat取自14歲男性供體外周血。該細(xì)胞源于DSMZ.

注釋

Group: Space-flown cell line (cellonaut).

Part of: Cancer Cell Line Encyclopedia (CCLE) project.

Part of: COSMIC cell lines project.

Part of: ENCODE project common cell types; tier 3.

Part of: International Histocompatibility Workshop cell lines.

Part of: LL-100 blood cancer cell line panel.

Doubling time: 26 hours (PubMed=17117183); ~25-35 hours (DSMZ).

Microsatellite instability: Instable (MSI-high) (PubMed=31068700; Sanger).

Omics: Acetylation analysis by proteomics.

Omics: Deep exome analysis.

Omics: Deep phosphoproteome analysis.

Omics: Deep proteome analysis.

Omics: Deep quantitative proteome analysis.

Omics: Deep RNAseq analysis.

Omics: DNA methylation analysis.

Omics: N-glycan profiling.

Omics: SNP array analysis.

Omics: Transcriptome analysis.

Omics: Ubiquitination analysis by proteomics.

Omics: Virome analysis using proteomics.

Anecdotal: Have been flown in space on Cosmos-2044 to study the inhibition of phorbol ester-induced cell activation in microgravity (PubMed=352912).

Misspelling: JURKET; In PubMed=313568.

Derived from sampling site: Peripheral blood.

基因突變

Heterozygous for BAX p.Glu41Argfs (c.121delG) and p.Glu41Glyfs (c.121dupG) (PubMed=9583678).

Heterozygous for FBXW7 p.Arg505Cys (c.1513C>T) (PubMed=22675565; Cosmic-CLP).

Heterozygous for INPP5D p.Gln345Ter (c.1033C>T) and c.1097+1065_1097+1112del47 (PubMed=19473701).

Homozygous for MSH2 p.Arg711Ter (c.2131C>T) (PubMed=9583678).

Homozygous for MSH6 p.Phe1088Serfs*2 (c.3261delC) (PubMed=9510473).

Heterozygous for NOTCH1 p.Arg1627His (c.4880G>A) (PubMed=15472075).

Heterozygous for TP53 p.Arg196Ter (c.586C>T) (PubMed=2144611; PubMed=10071127; PubMed=22675565; Cosmic-CLP).

HLA信息

/

STR信息

Amelogenin        X,Y

CSF1PO        9,10,11 (RCB_RCB0537)

10,11 (Cosmic-CLP; DSMZ)

10,12 (ECACC)

11 (RCB_RCB0806)

11,12 (CCRID; COG; PubMed=28357668; TKG)

D2S1338        19,23

D3S1358        15 (CCRID)

15,16,17 (COG)

15,17 (PubMed=28357668)

D5S818        9

D7S820        8,10 (CCRID; COG; ECACC; RCB_RCB0537; TKG)

8,10,11 (Cosmic-CLP; DSMZ; RCB_RCB0806)

8,12 (PubMed=28357668)

D8S1179        13,14 (CCRID; PubMed=28357668)

12,14,15 (COG)

D13S317        8,10,11 (TKG)

8,11 (COG; Cosmic-CLP; DSMZ; ECACC; RCB_RCB0537)

8,11,12 (RCB_RCB0806)

8,12 (CCRID; PubMed=28357668)

D16S539        10,11 (ECACC; RCB_RCB0537)

11 (CCRID; COG; Cosmic-CLP; DSMZ; PubMed=28357668; RCB_RCB0806; TKG)

D18S51        13,21

D19S433        13,15.2 (COG; PubMed=28357668)

14,15.2 (CCRID)

D21S11        31.2,32.2 (COG)

31.2,33.2 (CCRID; PubMed=28357668)

FGA        20,21 (CCRID; PubMed=28357668)

20,22 (COG)

Penta D        11,13

Penta E        10,12

TH01        6,9.3

TPOX        8,9,10 (RCB_RCB0537)

8,10 (CCRID; COG; Cosmic-CLP; DSMZ; ECACC; PubMed=28357668; RCB_RCB0806; TKG)

vWA        16,17,18 (RCB_RCB0537; RCB_RCB0806)

16,17,19,20 (ECACC)

17,18,19 (COG; DSMZ)

18 (CCRID; PubMed=28357668)

18,19,20 (Cosmic-CLP)

18,20 (TKG)

參考文獻(xiàn)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3

Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Nature 569:503-508(2019)

PubMed=31160637; DOI=10.1038/s41598-019-44491-x

Quentmeier H., Pommerenke C., Dirks W.G., Eberth S., Koeppel M., MacLeod R.A.F., Nagel S., Steube K., Uphoff C.C., Drexler H.G.

The LL-100 panel: 100 cell lines for blood cancer studies.

Sci. Rep. 9:8218-8218(2019)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023

Nusinow D.P., Szpyt J., Ghandi M., Rose C.M., McDonald E.R. III, Kalocsay M., Jane-Valbuena J., Gelfand E., Schweppe D.K., Jedrychowski M., Golji J., Porter D.A., Rejtar T., Wang Y.K., Kryukov G.V., Stegmeier F., Erickson B.K., Garraway L.A., Sellers W.R., Gygi S.P.

Quantitative proteomics of the Cancer Cell Line Encyclopedia.

Cell 180:387-402(2020)